Proteomics reveals the significance of vacuole Pi transporter in the adaptability of to Pi deprivation.

Vacuolar Pi transporters (VPTs) have recently been identified as important regulators of cellular Pi status in and . In the oil crop , and are two homologs of , the vacuolar Pi influx transporter in . Here, we show that Pi deficiency induces the transcription of both homologs of genes in leaves. double mutants (DM) had smaller shoots and higher cellular Pi concentrations than wild-type (WT, Westar 10), suggesting the potential role of in modulating cellular Pi status in . A proteomic analysis was performed to estimate the role of in Pi fluctuation. Results show that Pi deprivation disturbs the abundance of proteins in the physiological processes involved in carbohydrate metabolism, response to stimulus and stress in , while disruption of genes may exacerbate these processes. Besides, the processes of cell redox homeostasis, lipid metabolic and proton transmembrane transport are supposed to be unbalanced in DM under the -Pi condition. Noteworthy, disruption of genes severely alters the abundance of proteins related to ATP biosynthesis, and proton/inorganic cation transmembrane under normal Pi condition, which might contribute to growth limitations. Additionally, seven new protein markers of Pi homeostasis are identified in . Taken together, this study characterizes the important regulatory role of genes as vacuolar Pi influx transporters in Pi homeostasis in .