Toivio-Kinnucan M A, Chen H H, Li P H, Stushnoff C
Laboratory of Plant Hardiness, Department of Horticultural Science and Landscape Architecture, University of Minnesota, St. Paul, Minnesota 55108.
Plant Physiol. 1981 Mar;67(3):478-83. doi: 10.1104/pp.67.3.478.
Plasma membrane alterations in two tuber-bearing potato species during a 20-day cold acclimation period were investigated. Leaf-callus tissues of the frost-resistant Solanum acaule Hawkes ;Oka 3878' and the frost-susceptible, commonly grown Solanum tuberosum ;Red Pontiac,' were used. The former is a species that can be hardened after subjecting to the low temperature, and the latter does not harden. Samples for the electron microscopy were prepared from callus cultures after hardening at 2 C in the dark for 0, 5, 10, 15, and 20 days. After 20 days acclimation, S. acaule increased in frost hardiness from -6 to - 9 C (killing temperature), whereas frost hardiness of S. tuberosum remained unchanged (killed at -3 C). Actually, after 15 days acclimation, a -9 C frost hardiness level in S. acaule callus cultures had been achieved.Membrane protein particle aggregation was monitored using freeze-fracture electron microscopy. Protein particles were aggregated in S. acaule up to 10 days after the initiation of acclimation treatment and then redistributed almost to the level of control after 15 days. No such changes were observed for S. tuberosum under similar experimental conditions. The change in protein particle aggregation pattern in S. acaule is interpreted as indicating the presence of an adaptive fluidity control mechanism in that species.
研究了两种块茎类马铃薯在20天冷驯化期间质膜的变化。使用了抗冻的智利茄（Solanum acaule Hawkes；“Oka 3878”）和易受冻害、常见种植的马铃薯（Solanum tuberosum；“Red Pontiac”）的叶愈伤组织。前者是一种在经受低温后可以硬化的物种，而后者不会硬化。在黑暗中于2℃硬化0、5、10、15和20天后，从愈伤组织培养物中制备用于电子显微镜观察的样品。驯化20天后，智利茄的抗冻性从-6℃提高到-9℃（致死温度），而马铃薯的抗冻性保持不变（在-3℃时死亡）。实际上，驯化15天后，智利茄愈伤组织培养物已达到-9℃的抗冻水平。使用冷冻断裂电子显微镜监测膜蛋白颗粒的聚集。在驯化处理开始后的10天内，智利茄中的蛋白颗粒发生聚集，然后在15天后几乎重新分布到对照水平。在类似的实验条件下，马铃薯未观察到此类变化。智利茄中蛋白颗粒聚集模式的变化被解释为表明该物种存在适应性流动性控制机制。
Zotero 插件
