Department of Horticulture, Colorado State University, Fort Collins, Colorado 80523.
Plant Physiol. 1979 Jun;63(6):1220-2. doi: 10.1104/pp.63.6.1220.
Temperature dependence for partitioning of 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) between aqueous and lipid components of whole leaf tissue was measured. TEMPO is an electron spin resonance nitroxide label that has been used in model systems to detect membrane phase separations. Measurements were made on chilling-sensitive tomato leaves, frost-sensitive potato leaves, and frost-hardy and supercooled wheat leaves. The results suggest a membrane phase separation at 11 C in tomato, 3 C in potato, and -11 C in wheat.
测定了 2,2,6,6-四甲基哌啶-1-氧自由基（TEMPO）在整个叶片组织的水相和脂相之间分配的温度依赖性。TEMPO 是一种电子自旋共振氮氧自由基标记物，已被用于模型系统中以检测膜相分离。在对冷敏感的番茄叶片、对霜敏感的马铃薯叶片以及抗寒和过冷的小麦叶片上进行了测量。结果表明，在番茄中存在 11°C 的膜相分离，在马铃薯中存在 3°C 的膜相分离，在小麦中存在-11°C 的膜相分离。
