Laboratory of Plant Pathology, Faculty of Agriculture, Kyoto Prefectural University, Kyoto 606, Japan.
Plant Physiol. 1981 May;67(5):1032-5. doi: 10.1104/pp.67.5.1032.
A soluble elicitor of glyceollin accumulation was released from insoluble mycelial walls of Phytophthora megasperma var. sojae after incubation with soybean cotyledon tissue for as little as 2 minutes. Various enzymic and chemical treatments of the released elicitor indicated that the activity resided in a carbohydrate moiety, and gel filtration disclosed the presence of at least two active molecular species. Cell-free extracts from soybean cotyledons or hypocotyls also released soluble elicitors from fungal cell walls that were similar to those released by living cotyledon tissue. These results may suggest that contact of fungal pathogens with host tissues is required to release fungal wall elicitors which then initiate phytoalexin accumulation in the plant.
从感病大豆子叶组织孵育短短 2 分钟后,就可从大豆疫霉游动孢子游动孢子休止孢的不溶性菌壁中释放出一种可溶的甘油脂激发子。对所释放激发子的各种酶和化学处理表明,其活性存在于碳水化合物部分,凝胶过滤揭示至少存在两种有活性的分子种类。来自大豆子叶或下胚轴的无细胞提取物也可从真菌细胞壁中释放出类似于由活子叶组织释放的可溶激发子。这些结果可能表明,真菌病原体与宿主组织接触是必需的,以释放真菌细胞壁激发子,然后在植物中引发植保素的积累。