Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871 Copenhagen V, Denmark.
Plant Physiol. 1981 Oct;68(4):880-4. doi: 10.1104/pp.68.4.880.
Vanadate (Na(3) VO(4)) inhibits leaf movement and stomatal conductance of Phaseolus vulgaris L. cv. Carlos Favorit in light-dark cycles as well as photomorphogenetic leaf unrolling of Hordeum vulgare L. cv. Rupal. Inhibition was 50% by 10 to 100 micromolar vanadate and 100% by millimolar vanadate. Leaf unrolling was also inhibited by oligomycin and diethylstilbestrol.Millimolar vanadate is required to affect respiration of bean plants and barley leaves, while even this concentration has no effect on respiration of isolated pulvinal tissue.A nonspecific phosphatase activity extracted from both bean pulvini and barley leaves was found in all of four fractions of a differential centrifugation procedure (i.e. cell walls + nuclei, mitochondria, microsomes, super-natant), but more than 90% of the specific and total activity was located in the supernatant. The phosphatase activities of all fractions were inhibited by concentrations of vanadate similar to those which inhibited leaf movement, stomatal conductance of bean, and unrolling of barley leaves.
钒酸盐（Na(3) VO(4)）在光暗循环中抑制菜豆（Phaseolus vulgaris L. cv. Carlos Favorit）叶片运动和气孔导度，以及大麦（Hordeum vulgare L. cv. Rupal）的光形态建成叶片展开。10 至 100 微摩尔的钒酸盐抑制 50%，而毫摩尔的钒酸盐则完全抑制。寡霉素和己烯雌酚也抑制叶片展开。毫摩尔的钒酸盐会影响菜豆植物和大麦叶片的呼吸，但即使是这个浓度对分离的 pulvinal 组织的呼吸也没有影响。从菜豆 pulvini 和大麦叶片中提取的非特异性磷酸酶活性存在于差速离心过程的所有四个部分（即细胞壁+核、线粒体、微粒体、上清液）中，但特异性和总活性的 90%以上位于上清液中。所有部分的磷酸酶活性都被类似抑制叶片运动、豆科植物气孔导度和大麦叶片展开的钒酸盐浓度抑制。
