Poulson R, Beevers L
Department of Horticulture, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801.
Plant Physiol. 1970 Oct;46(4):509-14. doi: 10.1104/pp.46.4.509.
The influence of light, gibberellic acid, and abscisic acid on unrolling of etiolated barley leaf segments was investigated. Gibberellic acid stimulated unrolling of both illuminated and nonilluminated leaf segments. In contrast, abscisic acid prevented light-stimulated unrolling and abolished the slight unrolling of segments maintained in the dark.Analysis of the time course of photoinduced unrolling showed a lag period of 6 hours before the onset of rapid leaf unrolling. The application of gibberellic acid to illuminated segments shortened this lag phase. Also, incubating the segments in buffer in darkness prior to illumination eliminated the lag period. Inhibitors of RNA and protein synthesis prevented light- and gibberellic acid induced unrolling, indicating a requirement for macromolecular synthesis. The possibilities that the lag period of unrolling following illumination represents the time required for the removal of endogeneous inhibitor or the synthesis of essential macromolecules are discussed.
研究了光、赤霉素和脱落酸对黄化大麦叶片切段展开的影响。赤霉素促进光照和未光照叶片切段的展开。相比之下，脱落酸抑制光刺激的展开，并消除在黑暗中保存的切段的轻微展开。对光诱导展开的时间进程分析表明，在叶片快速展开开始前有6小时的滞后期。对光照切段施用赤霉素缩短了这个滞后期。此外，在光照前将切段在黑暗中的缓冲液中孵育可消除滞后期。RNA和蛋白质合成抑制剂阻止光和赤霉素诱导的展开，表明需要大分子合成。讨论了光照后展开滞后期代表去除内源性抑制剂或合成必需大分子所需时间的可能性。
