Department of Genetics, University of Helsinki, P. Rautatiekatu 13, 00100 Helsinki 10, Finland.
Plant Physiol. 1982 Jan;69(1):268-72. doi: 10.1104/pp.69.1.268.
The lipoxygenase (LOX) activity was determined in almost isogenic types of barley (Hordeum vulgare L.): normal cv. Adorra, cytoplasmic male sterile (msm1), and msm1 barley with restored fertility, heterozygous for the Rfm1a restorer gene. The LOX activity was lowest in male steriles in the leaf tissue studied at the anthesis stage. The LOX activity in developing anthers was higher than in leaf tissue, and decreased during degeneration of the sterile anthers.On polyacrylamide gel electrophoresis slabs, the LOX of anther homogenates moved in a complex which evidently carried some lipid and pigment, too. The LOX zones showed pseudoisoenzymic movement, i.e. a gradual increase in mobility dependent on the age of the anthers. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis slabs, the LOX zones contained three polypeptides. When sporopollenin production ends in the fertile anthers, a fourth polypeptide (molecular weight 91,200) appears in their LOX zones. This ;late' polypeptide is missing from steriles, and is suggested as being associated with the termination of sporopollenin production in the tapetum of fertiles.Sterile anthers were found to be almost devoid of soluble NH(2)-N, which supports the idea of their starvation. This starvation can reasonably be held responsible for the absence of late proteins (e.g. the 91,200 dalton polypeptide), and is reinforced by the uncontrolled production of sporopollenin.
脂氧合酶(LOX)活性在近等基因大麦(Hordeum vulgare L.)类型中测定:普通品种 Adorra、细胞质雄性不育(msm1)和 msm1 大麦,其雄性不育恢复育性,为 Rfm1a 恢复基因的杂合子。在开花期研究的叶组织中,雄性不育体的 LOX 活性最低。发育中的花药中的 LOX 活性高于叶组织,并且在不育花药退化过程中降低。在聚丙烯酰胺凝胶电泳板上,花药匀浆的 LOX 在显然携带一些脂质和色素的复杂体中移动。LOX 区显示假同工酶运动,即与花药年龄相关的迁移率逐渐增加。在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳板上,LOX 区含有三种多肽。当可育花药中的孢粉素生产结束时,其 LOX 区出现第四种多肽(分子量 91200)。这种“晚期”多肽在不育体中缺失,并被认为与可育体绒毡层中孢粉素生产的终止有关。不育花药中几乎没有可溶的 NH(2)-N,这支持了它们饥饿的观点。这种饥饿可以合理地归因于晚期蛋白(例如 91200 道尔顿多肽)的缺失,并受到孢粉素不受控制生产的加强。