生长素诱导下大豆下胚轴伸长区可翻译 mRNA 群体的变化。

Auxin-induced changes in the population of translatable messenger RNA in elongating sections of soybean hypocotyl.

机构信息

Department of Botany, University of Minnesota, St. Paul, Minnesota 55108.

出版信息

Plant Physiol. 1982 Feb;69(2):332-7. doi: 10.1104/pp.69.2.332.

DOI:10.1104/pp.69.2.332

PMID:16662203

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC426204/

Abstract

In vitro translation products of polyadenylated RNA from untreated and auxin-treated elongating sections of soybean (Glycine max var. Wayne) hypocotyl were analyzed by two-dimensional polyacrylamide gel electrophoresis. The levels of translatable messenger RNA for at least ten in vitro translation products are increased by auxin treatment. The induction by auxin occurs rapidly (within 15 minutes), and the amounts of the induced in vitro translation products increase with time of auxin treatment. Indoleacetic acid has the same effect on the population of translatable messenger RNA as 2,4-dichlorophenoxyacetic acid. The auxin-induced in vitro translation products disappear rapidly when Actinomycin D is present during the last two hours of a three-hour auxin treatment.

摘要

用二维聚丙烯酰胺凝胶电泳分析了未处理和生长素处理的大豆（Glycine max var. Wayne）下胚轴伸长区多聚腺苷酸化 RNA 的体外翻译产物。至少十种体外翻译产物的可翻译信使 RNA 的水平因生长素处理而增加。生长素的诱导作用迅速（在 15 分钟内），并且诱导的体外翻译产物的量随生长素处理时间的增加而增加。吲哚乙酸对可翻译信使 RNA 群体的作用与 2,4-二氯苯氧乙酸相同。当放线菌素 D 在三小时生长素处理的最后两小时存在时，生长素诱导的体外翻译产物迅速消失。