Plant Sciences Section, School of Agriculture and Forestry, University of Melbourne, Parkville, Victoria 3052, Australia.
Plant Physiol. 1982 Mar;69(3):575-9. doi: 10.1104/pp.69.3.575.
Protoplasts from 8- to 9-day-old wheat (Triticum aestivum L.) leaves were used to isolate organelles which were examined for their contents of peptide hydrolase enzymes and, in the case of vacuoles, other acid hydrolases. High yields of intact chloroplasts were obtained using both equilibrium density gradient centrifugation and velocity sedimentation centrifugation on sucrose-sorbitol gradients. Aminopeptidase activity was found to be distributed, in approximately equal proportions, between the chloroplasts and cytoplasm. Leucyltyrosine dipeptidase was mainly found in the cytoplasm, although about 27% was associated with the chloroplasts. Vacuoles shown to be free from Cellulysin contamination contained all of the protoplast carboxypeptidase and hemoglobin-degrading activities. The acid hydrolases, phosphodiesterase, acid phosphatase, alpha-mannosidase, and beta-N-acetylglucosamidase were found in the vacuole to varying degrees, but no beta-glucosidase was localized in the vacuole.
使用 8-9 天龄小麦（Triticum aestivum L.）叶片的原生质体分离细胞器，检查其肽水解酶的含量，以及液泡中其他酸性水解酶的含量。使用平衡密度梯度离心和蔗糖-山梨醇梯度上的速度沉降离心，可获得高产量的完整叶绿体。氨肽酶活性在叶绿体和细胞质中大致以相等的比例分布。亮氨酰酪氨酸二肽酶主要存在于细胞质中，尽管约 27%与叶绿体相关。表明不含纤维素酶污染的液泡含有所有原生质体羧肽酶和血红蛋白降解活性。磷酸二酯酶、酸性磷酸酶、α-甘露糖苷酶和β-N-乙酰葡萄糖胺酶等酸性水解酶在液泡中存在不同程度，但β-葡萄糖苷酶未定位在液泡中。
