Suntory Institute for Bioorganic Research, 1-1-1 Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka 618, Japan.
Plant Physiol. 1985 Nov;79(3):751-5. doi: 10.1104/pp.79.3.751.
Intracellular localization of lunularic acid and prelunularic acid in suspension cultured cells of Marchantia polymorpha L. was studied. The sum of both compounds was determined as lunularic acid group (LNAs) because of the instability of prelunularic acid to convert into lunularic acid.Mechanical disruption of the cells followed by differential centrifugation showed that LNAs was associated with the supernatant of 100,000g centrifugation. Protoplasts isolated from the cells were osmotically ruptured and the distribution of LNAs among the organelles was examined by discontinuous density gradient centrifugation of the protoplast contents. Successful isolation of intact chloroplasts, mitochondria and peroxisomes free from cytoplasm indicated that LNAs was not accumulated in these organelles. Flotation techniques resulted in an efficient isolation of pure vacuoles and revealed that LNAs was distributed almost equally in the vacuoles and cytoplasm.
研究了新月藻悬浮培养细胞中月桂烯酸和前月桂烯酸的细胞内定位。由于前月桂烯酸不稳定，会转化为月桂烯酸，因此将这两种化合物的总和确定为月桂烯酸组（LNAs）。细胞的机械破碎后进行差速离心显示，LNAs 与 100000g 离心的上清液有关。从细胞中分离出原生质体，通过原生质体内容物的不连续密度梯度离心检查 LNAs 在细胞器中的分布。成功分离出完整的叶绿体、线粒体和过氧化物酶体，没有细胞质，表明 LNAs 没有积累在这些细胞器中。浮选技术有效地分离出纯净的液泡，并揭示 LNAs 几乎均匀地分布在液泡和细胞质中。
