Biology Department, Queen's University, Kingston, Ontario Canada K7L 3N6.
Plant Physiol. 1982 Apr;69(4):825-8. doi: 10.1104/pp.69.4.825.
Ion filtration chromatography on diethylaminoethyl-Sephadex A-25 has been used to separate two isozymes each of triose phosphate isomerase, glyceraldehyde 3-phosphate dehydrogenase, glycerate 3-phosphate kinase, enolase, and phosphoglycerate mutase from homogenates of developing castor oil (Ricinus communis L. cv. Baker 296) seeds. Crude plastid fractions, prepared by differential centrifugation, were enriched in one of the isozymes, whereas the cytosolic fractions were enriched in the other. These data (and data published previously) indicate that plastids from developing castor oil seeds have a complete glycolytic pathway and are capable of conversion of hexose phosphate to pyruvate for fatty acid synthesis. The enzymes of this pathway in the plastids are isozymes of the corresponding enzymes located in the cytosol.
用二乙氨基乙基-葡聚糖 A-25 离子过滤层析法,从发育蓖麻油(Ricinus communis L. cv. Baker 296)种子的匀浆中分离出每个三磷酸甘油醛异构酶、甘油醛-3-磷酸脱氢酶、甘油酸-3-磷酸激酶、烯醇酶和磷酸甘油酸变位酶的两种同工酶。通过差速离心制备的粗质体部分富含一种同工酶,而胞质部分则富含另一种同工酶。这些数据(和以前发表的数据)表明,发育中的蓖麻油种子的质体具有完整的糖酵解途径,能够将己糖磷酸转化为丙酮酸用于脂肪酸合成。质体中该途径的酶是位于胞质溶胶中的相应酶的同工酶。