John Innes Institute, Colney Lane, NR4 7UH, Norwich, UK.
Planta. 1988 Feb;173(2):172-82. doi: 10.1007/BF00403008.
In order to determine whether the enzymes required to convert triose phosphate to acetyl CoA were present in pea (Pisum sativum L.) seed plastids, a rapid, mechanical technique was used to isolate plastids from developing cotyledons. The plastids were intact and the extraplastidial contamination was low. The following glycolytic enzymes, though predominantly cytosolic, were found to be present in plastids: glyceraldehyde 3-phosphate dehydrogenase (EC 1.2.1.12), phosphoglycerate kinase (EC 2.7.2.3), and pyruvate kinase(EC 2.7.1.40). Evidence is presented which indicates that plastids also contained low activities of enolase (EC 4.2.1.11) and phosphoglycerate mutase (EC 2.7.5.3). Pyruvate dehydrogenase, although predominantly mitochondrial, was also present in plastids. The plastidial activities of the above enzymes were high enough to account for the rate of lipid synthesis observed in vivo.
为了确定将三磷酸甘油醛转化为乙酰辅酶 A 所需的酶是否存在于豌豆（Pisum sativum L.）种子质体中，我们使用快速机械技术从发育中的子叶中分离质体。这些质体完整，质体外的污染很低。尽管以下糖酵解酶主要存在于细胞质中，但我们发现它们也存在于质体中：甘油醛 3-磷酸脱氢酶（EC 1.2.1.12）、磷酸甘油酸激酶（EC 2.7.2.3）和丙酮酸激酶（EC 2.7.1.40）。有证据表明，质体还含有低活性的烯醇酶（EC 4.2.1.11）和磷酸甘油酸变位酶（EC 2.7.5.3）。尽管丙酮酸脱氢酶主要存在于线粒体中，但它也存在于质体中。上述酶的质体活性足以解释体内观察到的脂质合成速率。
