研究参与藻类小球藻乙二醇代谢的氨基转移酶。
Studies on the aminotransferases participating in the glycolate metabolism of the alga mougeotia.
机构信息
Universität Oldenburg, Fachbereich Biologie, Postfach 2503, D-2900 Oldenburg, West Germany.
出版信息
Plant Physiol. 1982 Aug;70(2):340-3. doi: 10.1104/pp.70.2.340.
Abstract
Microbodies from Mougeotia spec., Strain 168.80 contain aminotransferases for conversion of glyoxylate to glycine and serine to hydroxypyruvate. Formation of glycine is possible at highest rates with alanine and glutamate as amino donors, whereas for deamination of serine, pyruvate and glyoxylate are the most convenient substrates. A serine hydroxymethyl-transferase was found exclusively in the mitochondrial fraction. There are indications that this enzyme is bound to the mitochondrial membranes. The activities of all transferases are increased under culture conditions stimulating the synthesis of glycolate.
摘要
来自 168.80 株的眉藻的微体含有转氨酶，可将乙醛酸转化为甘氨酸，丝氨酸转化为羟丙酮酸。用丙氨酸和谷氨酸作为氨基供体时，甘氨酸的生成速率最高，而丝氨酸脱氨时，丙酮酸和乙醛酸是最方便的底物。在线粒体部分发现了一种丝氨酸羟甲基转移酶。有迹象表明，这种酶与线粒体膜结合。在刺激甘醇酸合成的培养条件下，所有转移酶的活性都增加。
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