School of Life Sciences, Leicester Polytechnic, Leicester LE1 9BH United Kingdom.
Plant Physiol. 1983 Apr;71(4):927-31. doi: 10.1104/pp.71.4.927.
Sycamore (Acer pseudoplatanus L.) cell suspension cultures (strain OS) require 2,4-dichlorophenoxyacetic acid (2,4-D) in their culture medium for normal growth. If the 2,4-D is omitted, rates of cell division are dramatically reduced and cell lysis may occur. Despite this ;auxin requirement,' it has been shown by gas chromatography-mass spectrometry that the cells synthesize indol-3yl-acetic acid (IAA). Changes in free 2,4-D and IAA in the cells during a culture passage have been monitored.There is a rapid uptake of 2,4-D by the cells during the lag phase leading to a maximum concentration per cell (125 nanograms per 10(6) cells) on day 2 followed by a decline to 45 nanograms per 10(6) cells by day 9 (middle of linear phase). The initial concentration of IAA (0.08 nanograms per 10(6) cells) rises slowly to a peak of 1.4 nanograms per 10(6) cells by day 9 then decreases rapidly to 0.2 nanograms per 10(6) cells by day 15 (early declining phase) and 0.08 nanograms per 10(6) cells by day 23 (early stationary phase).
梧桐(Acer pseudoplatanus L.)细胞悬浮培养物(OS 株)在其培养基中需要 2,4-二氯苯氧乙酸(2,4-D)才能正常生长。如果省略 2,4-D,细胞分裂速度会显著降低,细胞可能会裂解。尽管存在“生长素需求”,但气相色谱-质谱联用仪已表明细胞合成吲哚-3-乙酸(IAA)。在培养过程中,细胞内游离 2,4-D 和 IAA 的变化已被监测到。在滞后期,细胞会迅速摄取 2,4-D,导致第 2 天每个细胞的最大浓度(10(6)个细胞中 125 纳克),然后在第 9 天(线性期中期)降至 45 纳克/10(6)个细胞。IAA 的初始浓度(10(6)个细胞中 0.08 纳克)缓慢上升,在第 9 天达到 1.4 纳克/10(6)个细胞的峰值,然后迅速下降到第 15 天(早期下降期)的 0.2 纳克/10(6)个细胞和第 23 天(早期静止期)的 0.08 纳克/10(6)个细胞。