D.R.F., Biologie Végétale, Centre d'Etudes Nucléaires de Grenoble, 85 X, 38041 Grenoble Cedex, France.
Plant Physiol. 1977 Mar;59(3):502-5. doi: 10.1104/pp.59.3.502.
A phytostat to mass culture higher plant cells in liquid medium is described. This apparatus allowed the culture in batch, turbidostat and chemostat of 20 liters of cells. Automatic control of cell suspension growth was based on culture turbidity. Changes with time of certain cell characteristics, particularly cell respiration and phospholipid content, indicated that the test time to harvest large amounts of sycamore cells (Acer pseudoplatanus L.) in good physiological state was about 2 days before the end of the exponential phase of growth, when the cell density reached one million cells per milliliter of culture.
一种用于在液体培养基中大规模培养高等植物细胞的植物生长调节剂被描述。该设备允许在批式、恒浊和恒化培养中培养 20 升细胞。细胞悬浮液生长的自动控制基于培养物浊度。某些细胞特性随时间的变化，特别是细胞呼吸和磷脂含量的变化，表明在生长指数期结束前约 2 天，当细胞密度达到每毫升培养物 100 万个细胞时，收获大量梧桐细胞（Acer pseudoplatanus L.）的最佳生理状态的测试时间。
