School of Botany, Trinity College, University of Dublin, Dublin 2, Eire.
Plant Physiol. 1983 May;72(1):22-5. doi: 10.1104/pp.72.1.22.
The chromatographic properties of glutamine synthetase isoforms have been investigated in a wide range of higher plant leaves and shoots using ion exchange chromatography. Different patterns of glutamine synthetase isoform content were observed. Among higher plants, four patterns or groups could be recognized. The first group is characterized by having only cytosolic glutamine synthetase, whereas the second group is distinguished by having only chloroplastic glutamine synthetase. The third group is characterized by cytosolic glutamine synthetase being a minor component of the total leaf glutamine synthetase activity. The fourth group is distinct from the other groups in having high cytosolic and chloroplast glutamine synthetase activity. Immunological studies have been undertaken on a few species from each group to identify unambiguously both cytosolic and chloroplastic glutamine synthetases.
采用离子交换层析法研究了多种高等植物叶片和茎中谷氨酰胺合成酶同工酶的色谱性质。观察到不同的谷氨酰胺合成酶同工酶含量模式。在高等植物中，可以识别出四种模式或组。第一组的特征是仅具有胞质谷氨酰胺合成酶，而第二组的特征是仅具有叶绿体谷氨酰胺合成酶。第三组的特征是胞质谷氨酰胺合成酶是总叶片谷氨酰胺合成酶活性的次要组成部分。第四组与其他组的区别在于具有高的胞质和叶绿体谷氨酰胺合成酶活性。已经对每组中的少数几个物种进行了免疫学研究，以明确鉴定胞质和叶绿体谷氨酰胺合成酶。
