Department of Biochemistry, University of Missouri, Columbia, Missouri 65212.
Plant Physiol. 1983 May;72(1):262-3. doi: 10.1104/pp.72.1.262.
Soybeans (Glycine max L. Merr. cv ;Maple Presto') harvested from plants cultured in nickel-free medium had <0.005% the activity of nickel-sufficient beans and only 15% the activity of a urease-null variety, Itachi. However, whereas Itachi has no detectable urease protein, nickel-free beans of the variety Maple Presto exhibit normal or near normal levels of urease apoprotein. Thus, nickel isn't necessary for urease apoprotein synthesis. The apoprotein wasn't activated by nickel in vitro but, upon seed imbibition of nickel, urease was partially activated. This in vivo activation was not inhibited by cychoheximide.
从在无镍培养基中培养的植株上收获的大豆（Glycine max L. Merr. cv; Maple Presto'），其活性比镍充足的大豆低<0.005%，仅为尿酶缺失品种 Itachi 的 15%。然而，虽然 Itachi 没有检测到尿酶蛋白，但 Maple Presto 品种的无镍豆表现出正常或接近正常水平的尿酶辅基蛋白。因此，镍不是尿酶辅基蛋白合成所必需的。该辅基蛋白在体外不能被镍激活，但在种子吸镍后，尿酶被部分激活。这种体内激活不受环己酰亚胺抑制。
