大麦群体中核酮糖-1,5-二磷酸羧化酶反应动力学的变异性。
Variability of Reaction Kinetics for Ribulose-1,5-bisphosphate Carboxylase in a Barley Population.
机构信息
Department of Botany and Range Science, Brigham Young University, Provo, Utah 84602.
出版信息
Plant Physiol. 1983 May;72(1):76-9. doi: 10.1104/pp.72.1.76.
The photosynthetic enzyme ribulose bisphosphate carboxylase-oxygenase [EC 4.1.1.39] (RuBPCase) plays a key role in the carbon reduction system of plants. In this study, we determined the kinetic variability of RuBPCase among 46 varieties of Hordeum vulgare L. at two ages. The V(max) CO(2) and K(m) CO(2) of RuBPCase was determined for each cultivar. Varietal differences were found in K(m) CO(2) and V(max) CO(2) for one and four genotypes, respectively. One variety exhibited atypical behavior in both K(m) and V(max). A comparison of varieties and age showed a significant interaction between these factors for K(m) but not for V(max). These data indicate the presence of kinetic variability in RuBPCase within the H. vulgare population and perhaps between plant ages.
光合作用酶核酮糖-1,5-二磷酸羧化酶/加氧酶[EC 4.1.1.39]（RuBPCase）在植物的碳还原系统中起着关键作用。在这项研究中，我们在两个年龄阶段测定了 46 个大麦品种中 RuBPCase 的动力学变异性。测定了每个品种的 RuBPCase 的最大 CO2 比活性（V(max)CO2）和 CO2 米氏常数（K(m)CO2）。分别在 1 个和 4 个基因型中发现了 K(m)CO2 和 V(max)CO2 的品种差异。一个品种在 K(m)和 V(max)方面都表现出非典型行为。品种和年龄的比较表明，K(m)之间存在显著的相互作用，但 V(max)之间没有相互作用。这些数据表明，在大麦种群中存在 RuBPCase 的动力学变异性，也许还存在于不同的植物年龄之间。
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