Department of Botany and Plant Sciences, University of California, Riverside, CA 92521.
Plant Physiol. 1983 Jul;72(3):611-5. doi: 10.1104/pp.72.3.611.
Xerosicyos danguyi Humbert (Cucurbitaceae) is a leaf succulent endemic to Madagascar. Under well-watered conditions, the plant exhibited Crassulacean acid metabolism (CAM) but shifted to a dampened form of CAM, CAM-idling, when subjected to water stress. The purpose of this investigation was to examine the effects of a shift in carbon metabolism on phosphoenolpyruvate carboxylase and on NADP-malic enzyme in X. danguyi. Experiments were conducted to determine the diurnal patterns of enzyme activity and pH optima of both enzymes, as well as the approximate molecular mass, kinetic patterns, malate inhibition, and glucose-6-phosphate stimulation of phosphoenolpyruvate carboxylase. The two enzymes extracted from well-watered and water-stressed plants were similar in most parameters investigated; thus, CAM-idling appeared to be only a dampened form of CAM photosynthesis.
马达加斯加特有物种黄丝吊蝴蝶(葫芦科)是一种叶肉质植物。在水分充足的条件下,该植物表现出景天酸代谢(CAM),但在受到水分胁迫时,会转变为一种减弱形式的 CAM——CAM 休眠。本研究旨在探讨碳代谢转变对黄丝吊蝴蝶的磷酸烯醇丙酮酸羧化酶和 NADP-苹果酸酶的影响。实验测定了这两种酶的酶活性日变化模式以及最适 pH 值,还测定了磷酸烯醇丙酮酸羧化酶的近似分子量、动力学模式、苹果酸抑制作用以及葡萄糖-6-磷酸刺激作用。从水分充足和水分胁迫的植株中提取的这两种酶在大多数研究参数上相似;因此,CAM 休眠似乎只是 CAM 光合作用的一种减弱形式。