Department of Biochemistry, University of Georgia, Athens, Georgia 30602.
Plant Physiol. 1973 Oct;52(4):357-61. doi: 10.1104/pp.52.4.357.
Phosphoenolpyruvate carboxykinase has been found in significant activities in a number of plants exhibiting Crassulacean acid metabolism. Thirty-five species were surveyed for phosphoenolpyruvate carboxykinase, phosphoenolpyruvate carboxylase, ribulose diphosphate carboxylase, malic enzyme, and malate dehydrogenase (NAD). Plants which showed high activities of malic enzyme contained no detectable phosphoenolpyruvate carboxykinase, while plants with high activities of the latter enzyme contained little malic enzyme. It is proposed that phosphoenolpyruvate carboxykinase acts as a decarboxylase during the light period, furnishing CO(2) for the pentose cycle and phosphoenolpyruvate for gluconeogenesis.Some properties of phosphoenolpyruvate carboxykinase in crude extracts of pineapple leaves were investigated. The enzyme required Mn(2+), Mg(2+), and ATP for maximum activity. About 60% of the activity could be pelleted, along with chloroplasts and mitochondria, in extracts from leaves kept in the dark overnight.
磷酸烯醇式丙酮酸羧激酶在具有景天酸代谢的多种植物中发现具有显著的活性。对 35 种植物的磷酸烯醇式丙酮酸羧激酶、磷酸烯醇式丙酮酸羧化酶、核酮糖二磷酸羧化酶、苹果酸酶和苹果酸脱氢酶（NAD）进行了调查。表现出苹果酸酶高活性的植物中没有检测到可检测的磷酸烯醇式丙酮酸羧激酶，而具有高活性后者酶的植物中苹果酸酶含量很少。据提议，磷酸烯醇式丙酮酸羧激酶在光照期间作为脱羧酶发挥作用，为戊糖循环和磷酸烯醇式丙酮酸的糖异生提供 CO2。对菠萝叶粗提物中的磷酸烯醇式丙酮酸羧激酶的一些性质进行了研究。该酶需要 Mn(2+)、Mg(2+) 和 ATP 才能达到最大活性。在叶片提取物中，约 60%的活性可与叶绿体和线粒体一起沉淀，这些叶片在黑暗中放置过夜。
