Gruppo di Chimica Biologica e Strutturistica Chimica, Istituto di Chimica Biologica, Università degli Studi di Roma, 00185 Roma Italy.
Plant Physiol. 1983 Sep;73(1):182-4. doi: 10.1104/pp.73.1.182.
Ferricyanide is actively reduced by intact maize (Zea mays L., var XL 342) roots. This reduction is salt and temperature dependent, is stimulated by fusicoccin, and is accompanied by decrease of external pH. In anaerobic conditions, ferricyanide partially restores fusicoccin-induced proton extrusion. A salt-, temperature-, and pH-dependent cyanide-insensitive NADH-ferricyanide oxidoreductase activity can be demonstrated in microsomes isolated from the same plant tissue. This evidence supports the hypothesis, as proposed by Craig and Crane (1982 Plant Physiol 67: S-558, S-835), that the ferricyanide reduction is carried out by a transmembrane NADH dehydrogenase.
亚铁氰化物可被完整的玉米(Zea mays L.,变种 XL 342)根系主动还原。这种还原作用依赖于盐和温度,被 fusicoccin 刺激,并伴随着外部 pH 值的降低。在厌氧条件下,亚铁氰化物部分恢复 fusicoccin 诱导的质子外排。可以从相同的植物组织分离的微粒体中证明盐、温度和 pH 依赖性、氰化物不敏感的 NADH-亚铁氰化物氧化还原酶活性。这一证据支持了 Craig 和 Crane(1982 年《植物生理学》67:S-558,S-835)提出的假说,即亚铁氰化物的还原是由跨膜 NADH 脱氢酶进行的。