巴西橡胶树胶乳中类液泡体膜的电子传递

Electron transport in the membrane of lutoids from the latex of Hevea brasiliensis.

Moreau F, Jacob J L, Dupont J, Lance C

Biochim Biophys Acta. 1975 Jul 8;396(1):116-24. doi: 10.1016/0005-2728(75)90194-2.

Abstract

An antimycin-insensitive NADH-cytochrome c oxidoreductase (E.C. 1.6.99.3) activity can be demonstrated in the membrane of lutoids isolated from the latex of Hevea brasiliensis. This electron transport system can also use ferricyanide as an electron acceptor, but is unable to oxidize NADPH. 2. Two beta-type cytochromes are present in the membranes. Cytochrome beta563 is partially reduced by NADH and ascorbate, but is not reducible by NADPH. It shows a double peak at 555 and 561 nm at 77 degrees K. A second cytochrome, cytochrome beta561, seems to be reducible by hydrosulfite only. 3. In the reduced state, these cytochromes do not combine with CO. The occurrence of cytochrome P-450 could not be demonstrated. 4. The role of the NADH oxidation system is considered in relation to the biosynthesis of polyisoprene compounds in the latex.

摘要

从巴西橡胶树胶乳中分离得到的 lutoids 膜中可检测到一种抗霉素不敏感的 NADH - 细胞色素 c 氧化还原酶（E.C. 1.6.99.3）活性。该电子传递系统也能利用铁氰化物作为电子受体，但不能氧化 NADPH。2. 膜中存在两种β型细胞色素。细胞色素β563 可被 NADH 和抗坏血酸部分还原，但不能被 NADPH 还原。在 77K 时，它在 555 和 561nm 处呈现双峰。第二种细胞色素，细胞色素β561，似乎仅能被连二亚硫酸盐还原。3. 在还原状态下，这些细胞色素不与 CO 结合。未检测到细胞色素 P - 450 的存在。4. 结合胶乳中聚异戊二烯化合物的生物合成，对 NADH 氧化系统的作用进行了探讨。