Northern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, Peoria, Illinois 61604.
Plant Physiol. 1983 Sep;73(1):51-5. doi: 10.1104/pp.73.1.51.
Pigment-protein complexes were isolated from chloroplasts of normal green and several types of chlorophyll-deficient soybeans. The complexes were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and comparisons were made between normal and chlorophyll-deficient genotypes of the relative amounts of chlorophyll associated with Photosystem I (PSI), Photosystem II (PSII), light-harvesting, and free pigment complexes.Chlorophyll-deficient genotypes, compared to normal green genotypes, have fewer light-harvesting complexes and a higher ratio of PSII to PSI complexes. Chlorophyll associated with PSII in yellow genotypes is in relatively higher amounts in spite of the fact that these genotypes have much less grana stacking than normal green genotypes. Although PSII activity has been associated with appressed regions of grana in normal plants, our work shows that the association does not always hold true.
从正常绿色和几种叶绿素缺乏型大豆的叶绿体中分离出了色素-蛋白复合物。这些复合物通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行分离,并比较了正常和叶绿素缺乏型基因型之间与光系统 I(PSI)、光系统 II(PSII)、光捕获和游离色素复合物相关的叶绿素的相对含量。与正常绿色基因型相比,叶绿素缺乏型基因型的光捕获复合物较少,PSII 与 PSI 复合物的比例较高。尽管黄色基因型的类囊体堆叠比正常绿色基因型少得多,但与 PSII 结合的叶绿素含量相对较高。尽管 PSII 活性与正常植物的类囊体压紧区域有关,但我们的工作表明,这种关联并不总是成立。