The Ohio State University, Department of Physiological Chemistry, Columbus, Ohio 43210.
Plant Physiol. 1983 Nov;73(3):858-9. doi: 10.1104/pp.73.3.858.
Soybean (Glycine soja var Beeson) formate dehydrogenase has been isolated, purified, and partially characterized by affinity chromatography. The enzyme is a dimer having a total molecular weight of 100,000 and a subunit weight of 47,000. It has activity over a broad pH range, is stable for months at 4 degrees C, and has K(m) values of 0.6 millimolar and 5.7 micromolar for formate and NAD, respectively.
大豆(Glycine soja var Beeson)甲酸盐脱氢酶已通过亲和层析法进行了分离、纯化和部分特性鉴定。该酶为二聚体,总分子量为 100,000,亚基分子量为 47,000。它在较宽的 pH 范围内具有活性,在 4°C 下稳定数月,其对甲酸盐和 NAD 的 K(m)值分别为 0.6 毫摩尔和 5.7 微摩尔。