National Institute for Basic Biology, Myodaijicho, Okazaki, Japan 444.
Plant Physiol. 1984 Feb;74(2):268-71. doi: 10.1104/pp.74.2.268.
Effects of the calcium ionophore, A23187, and antimycin A on the circadian conidiation rhythm of Neurospora crassa were examined. A23187 at a concentration of 1 mum in medium not containing divalent cations delayed the phase by 10 hours at CT 10 and advanced it by 5 hours at CT 14 (CT 12 corresponds to the time that discs are transferred from light to dark). This phase shifting was completely inhibited by addition of 0.1 millimolar CaCl(2) but not by MgCl(2) at any concentrations examined.Antimycin A inhibited respiration by 90% at a concentration of 0.2 micrograms per milliliter and lowered the ATP content by 85%. Antimycin A alone caused small phase advances but in combination with A23187 resulted in a large phase delay at CT 10. This phase shifting was not reversed by addition of CaCl(2) lower than 10 millimolar.
研究了钙离子载体 A23187 和抗霉素 A 对粗糙脉孢菌的昼夜分生孢子形成节律的影响。在不含二价阳离子的培养基中,浓度为 1 mum 的 A23187 在 CT 10 时将相位延迟 10 小时,在 CT 14 时提前 5 小时(CT 12 对应于将圆盘从光照转移到黑暗的时间)。这种相移完全被添加 0.1 毫摩尔氯化钙抑制,但在任何检查的浓度下均不受 MgCl2 抑制。抗霉素 A 在浓度为 0.2 微克/毫升时抑制呼吸 90%,并将 ATP 含量降低 85%。抗霉素 A 单独引起小的相位提前,但与 A23187 结合在 CT 10 时导致大的相位延迟。相移不能通过添加低于 10 毫摩尔的氯化钙来逆转。