National Institute for Basic Biology, Myodaijicho, Okazaki 444, Japan.
Plant Physiol. 1982 Oct;70(4):982-6. doi: 10.1104/pp.70.4.982.
Phase shifts of the circadian conidiation rhythm in Neurospora crassa were induced by 3-hour treatments of mycelia in liquid medium with diethylstilbestrol (DES), dienestrol (DIE), hexestrol (HEX), diethylstilbestroldipropionate (DESP), and dienestroldiacetate (DIEA). Over a 24-hour period beginning 24 hours after the transition from light to constant dark, maximum phase shifts occurred about 36 hours. DES was the most effective of the drugs tested, giving 10-hour phase advances at 20 micromolar. DIE and HEX caused similar phase shifts as DES at 40 micromolar. The two derivatives of the last, DESP and DIEA, were much less effective in shifting phase; only a few hours of phase advance result from treatments at 80 micromolar concentrations.The activity of isolated plasma membrane ATPase was inhibited by DES and partially by HEX, but not by DIE, DESP, or DIEA. O(2) consumption of the mycelia was inhibited equally by DES, DIE, and HEX, while DIEA and DESP had little effect. Phase-shifts by DES cannot be interpreted as evidence that plasma membrane ATPase is a component of the circadian clock.
在Neurospora crassa 中,用二乙基己烯雌酚(DES)、己烯雌酚(DIE)、己烷雌酚(HEX)、二乙基己烯雌酚二丙酸酯(DESP)和己烯雌酚二乙酸酯(DIEA)对菌丝体进行 3 小时处理,可诱导昼夜节律的分生孢子形成节奏的相移。在从光照到持续黑暗的转变后 24 小时开始的 24 小时周期内,最大的相移发生在大约 36 小时。DES 是测试的药物中最有效的,在 20 微摩尔时可产生 10 小时的相移提前。DIE 和 HEX 在 40 微摩尔时与 DES 引起相似的相移。最后两种物质的两种衍生物,DESP 和 DIEA,在相移方面的效果要差得多;在 80 微摩尔浓度的处理下,只有几个小时的相移提前。DES 和部分 HEX 抑制了分离的质膜 ATP 酶的活性,但 DIE、DESP 或 DIEA 则没有。DES、DIE 和 HEX 同样抑制了菌丝体的 O(2)消耗,而 DIEA 和 DESP 的影响则较小。DES 的相移不能解释为质膜 ATP 酶是昼夜节律钟的组成部分的证据。