在蓝藻鱼腥藻中的体外脂肪酸合成和复杂脂质代谢：二。酰基转移和复杂脂质的形成。
In Vitro Fatty Acid Synthesis and Complex Lipid Metabolism in the Cyanobacterium, Anabaena Variabilis: II. Acyl Transfer and Complex Lipid Formation.
机构信息
Department of Biochemistry & Biophysics, University of California, Davis, California 95616.
出版信息
Plant Physiol. 1984 Jul;75(3):700-4. doi: 10.1104/pp.75.3.700.
In vitro fatty acid transfer to form complex lipids was observed in crude cell extracts of Anabaena variabilis using [1-(14)C]palmitoyl-acyl carrier protein, [1-(14)C]stearoyl-acyl carrier protein, and [1-(14)C]oleoyl-acyl carrier protein substrates. The data indicated that there was a rapid transfer of the fatty acids into the complex lipids. The greatest amount of radioactivity was observed in the monogalactosyl diacylglycerol fractions and there appeared to be a preference for the transfer of stearate over palmitate. The exogenously added lysophospholipids, (lysophosphatidylglycerol, lysophosphatidylcholine) and 2-monopalmitin acted as acceptors in acyl transfer. Addition of the hypolipidemic drug, WY14643, inhibited the fast acyl transfer reaction and showed that the first product of acyl transfer was diglyceride followed by monogalactosyl diacylglycerol. Thioesters of Coenzyme A do not seem to be involved in these reactions.
在变鱼腥藻的粗细胞提取物中用[1-(14)C]棕榈酰酰基载体蛋白、[1-(14)C]硬脂酰酰基载体蛋白和[1-(14)C]油酰酰基载体蛋白底物观察到脂肪酸向复合脂质的体外转移。数据表明，脂肪酸迅速转移到复合脂质中。在单半乳糖基二酰甘油馏分中观察到最大量的放射性，并且似乎存在硬脂酸对棕榈酸的转移偏好。外源性添加的溶血磷脂，（溶血磷脂酰甘油、溶血磷脂酰胆碱）和 2-单棕榈酸作为酰基转移的受体。添加降脂药物 WY14643 抑制快速酰基转移反应，并表明酰基转移的第一个产物是二酰甘油，然后是单半乳糖基二酰甘油。辅酶 A 的硫酯似乎不参与这些反应。
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