Lem N W, Stumpf P K
Department of Biochemistry and Biophysics, University of California, Davis, California 95616.
Plant Physiol. 1984 Jan;74(1):134-8. doi: 10.1104/pp.74.1.134.
In vitro fatty acid synthesis was examined in crude cell extracts, soluble fractions, and 80% (NH(4))(2)SO(4) fractions from Anabaena variabilis M3. Fatty acid synthesis was absolutely dependent upon acyl carrier protein and required NADPH and NADH. Moreover, fatty acid synthesis and elongation occurred in the cytoplasm of the cell. The major fatty acid products were palmitic acid (16:0) and stearic acid (18:0). Of considerable interest, both stearoyl-acyl carrier protein and stearoyl-coenzyme A desaturases were not detected in any of the fractions from A. variabilis. The similarities and differences in fatty acid synthesis between A. variabilis and higher plant tissues are discussed with respect to the endosymbiotic theory of chloroplast evolution.
对多变鱼腥藻M3的粗细胞提取物、可溶性组分和80%硫酸铵组分中的体外脂肪酸合成进行了检测。脂肪酸合成绝对依赖于酰基载体蛋白,且需要NADPH和NADH。此外,脂肪酸合成和延伸发生在细胞的细胞质中。主要的脂肪酸产物是棕榈酸(16:0)和硬脂酸(18:0)。值得注意的是,在来自多变鱼腥藻的任何组分中均未检测到硬脂酰-酰基载体蛋白和硬脂酰辅酶A去饱和酶。关于叶绿体进化的内共生理论,讨论了多变鱼腥藻与高等植物组织在脂肪酸合成方面的异同。