Biological Sciences Group U-42, University of Connecticut, Storrs, Connecticut 06268.
Plant Physiol. 1984 Nov;76(3):685-90. doi: 10.1104/pp.76.3.685.
Concentrations of K, Cl, P, S, and Ca in extensor and flexor protoplasts from open pulvini of the nyctinastic tree Samanea saman were estimated using x-ray microanalysis. This technique is particularly suitable when absolute numbers of protoplasts are low, because less than 100 protoplasts are required to obtain statistically significant data. Flexor protoplasts contain similar concentrations of P and S but almost twice as much K and Cl as extensor protoplasts. Low levels of total measurable osmoticum suggest that extensive leakage has occurred during protoplast isolation. Both extensor and flexor protoplasts appear to contain some unidentified osmoticum not detectable by x-ray analysis. Extensor protoplasts must have more unidentified osmoticum to compensate for their lower levels of K and Cl.
利用 X 射线微量分析技术测定了含羞树（Samanea saman）昼开夜合叶的外展和内弯原质体中 K、Cl、P、S 和 Ca 的浓度。当原质体数量较少时，该技术特别适用，因为只需不到 100 个原质体就可获得具有统计学意义的数据。内弯原质体中 P 和 S 的浓度相似，但 K 和 Cl 的浓度几乎是外展原质体的两倍。可测量的总渗透压水平较低表明，在原质体分离过程中发生了广泛的渗漏。外展和内弯原质体似乎都含有一些无法通过 X 射线分析检测到的未知渗透压物质。外展原质体必须含有更多未知的渗透压物质来补偿其较低的 K 和 Cl 水平。
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