School of Agriculture, La Trobe University, Bundoora, Victoria, 3083, Australia.
Plant Physiol. 1987 Oct;85(2):331-4. doi: 10.1104/pp.85.2.331.
Phosphorus contents of epidermal vacuoles and mesophyll cells of Lupinus lutens leaflets were measured by electron probe x-ray microanalysis of fully hydrated, bulk frozen samples. Quantitation was achieved using standard solutions containing colloidal graphite to simulate cell contents and the derived nonlinear relationship between peak over background ratio and concentration improved the accuracy of the analytical procedure. Inorganic phosphate contents of mesophyll cells were shown to be highly dependent on phosphate nutrition. Comparison with data obtained from conventional analysis leads to the suggestion that in heterogeneous tissues the inorganic phosphate concentrations of the cytoplasm may show greater variation than observed in the cytoplasm of simple plant systems such as cell suspension and root tips.
采用电子探针 X 射线微量分析技术，对充分水合、整体冷冻的样品进行分析，测定了羽扇豆小叶表皮液泡和叶肉细胞的磷含量。通过含有胶体石墨的标准溶液进行定量分析，模拟细胞内容物，得出了峰背景比与浓度之间的非线性关系，从而提高了分析程序的准确性。结果表明，叶肉细胞的无机磷含量高度依赖于磷营养。与常规分析获得的数据进行比较，表明在不均匀组织中，细胞质的无机磷浓度可能比悬浮细胞和根尖等简单植物系统的细胞质中的变化更大。
