铁氧还蛋白对蓝细菌铁氧还蛋白-NADP 还原酶的黄递酶活性的影响。
Effect of Ferredoxin on the Diaphorase Activity of Cyanobacterial Ferredoxin-NADP Reductase.
机构信息
The Hebrew University of Jerusalem, Faculty of Agriculture, Rehovot, Israel.
出版信息
Plant Physiol. 1985 Jan;77(1):229-31. doi: 10.1104/pp.77.1.229.
Abstract
The interaction of ferredoxin-NADP reductase (FNR) and ferredoxin (Fd) results in an enhanced rate of reaction and a shift of the pH optimum for the FNR-mediated diaphorase reaction. Low concentrations of NaCl (<100 millimolar), favorable for formation of the FNR:Fd complex, further magnify the alteration of the diaphorase reaction; the activity is enhanced 3-fold and pH optimum is shifted from 9.5 to 7.8. The Fd-stimulated diaphorase activity of FNR may result either from a conformational change of the enzyme and/or from a transition from a two electron to a one electron reaction.
摘要
铁氧还蛋白-NADP 还原酶（FNR）与铁氧还蛋白（Fd）的相互作用可提高反应速率，并使 FNR 介导的黄递酶反应的 pH 最佳值发生偏移。低浓度的 NaCl（<100 毫摩尔）有利于 FNR:Fd 复合物的形成，进一步放大黄递酶反应的变化；酶活性增强 3 倍，pH 最佳值从 9.5 偏移至 7.8。FNR 的 Fd 刺激的黄递酶活性可能是由于酶的构象变化和/或从两电子反应转变为一电子反应。
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