Department of Biochemistry and Genetics, The Connecticut Agricultural Experiment Station, 123 Huntington Street, P.O. Box 1106, New Haven, Connecticut 06504.
Plant Physiol. 1985 Jan;77(1):240-2. doi: 10.1104/pp.77.1.240.
Organic gelling agents such as agar and agarose provide a heterotrophic substrate for growth of illuminated tobacco callus. When green cells are incubated in CO(2)-free air on a medium lacking sucrose but solidified with 1% agar, an increase in relative dry weight is sustained through two passages. Similar results with different inoculum sources, and with three brands of agar and two forms of agarose, suggest this is a general phenomenon. A fully autotrophic culture system was developed employing polyurethane pads to support cells in a liquid medium lacking sucrose. Growth was negligible in two passages in CO(2)-free air, and increased with each added increment in CO(2) concentration.
有机胶凝剂，如琼脂和琼脂糖，为光照培养的烟草愈伤组织提供异养基质。当绿色细胞在缺乏蔗糖但用 1%琼脂固化的 CO(2)自由空气中孵育时，通过两次传代可以维持相对干重的增加。不同接种源和三种琼脂及两种琼脂糖的相似结果表明，这是一种普遍现象。开发了一种完全自养培养系统，采用聚氨酯垫在缺乏蔗糖的液体培养基中支撑细胞。在 CO(2)自由空气中进行两次传代时，生长可以忽略不计，并且随着 CO(2)浓度的每次增加而增加。
