严格自养培养烟草愈伤组织的条件。
Conditions for strict autotrophic culture of tobacco callus.
机构信息
Department of Biochemistry and Genetics, The Connecticut Agricultural Experiment Station, 123 Huntington Street, P.O. Box 1106, New Haven, Connecticut 06504.
出版信息
Plant Physiol. 1985 Jan;77(1):240-2. doi: 10.1104/pp.77.1.240.
Organic gelling agents such as agar and agarose provide a heterotrophic substrate for growth of illuminated tobacco callus. When green cells are incubated in CO(2)-free air on a medium lacking sucrose but solidified with 1% agar, an increase in relative dry weight is sustained through two passages. Similar results with different inoculum sources, and with three brands of agar and two forms of agarose, suggest this is a general phenomenon. A fully autotrophic culture system was developed employing polyurethane pads to support cells in a liquid medium lacking sucrose. Growth was negligible in two passages in CO(2)-free air, and increased with each added increment in CO(2) concentration.
有机胶凝剂，如琼脂和琼脂糖，为光照培养的烟草愈伤组织提供异养基质。当绿色细胞在缺乏蔗糖但用 1%琼脂固化的 CO(2)自由空气中孵育时，通过两次传代可以维持相对干重的增加。不同接种源和三种琼脂及两种琼脂糖的相似结果表明，这是一种普遍现象。开发了一种完全自养培养系统，采用聚氨酯垫在缺乏蔗糖的液体培养基中支撑细胞。在 CO(2)自由空气中进行两次传代时，生长可以忽略不计，并且随着 CO(2)浓度的每次增加而增加。
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