Biology Department, McMaster University, Hamilton, Ontario, Canada L8S 4K1.
Plant Physiol. 1985 Mar;77(3):520-3. doi: 10.1104/pp.77.3.520.
The fate of glutamine, the major source of nitrogen supplied to the developing maize endosperm, has been examined in endosperm tissues of corn caryopsis grown under sterile conditions. In the culture system, [U-(14)C]glutamine was included in the medium or was injected directly into the endosperm. Samples were harvested at intervals up to 168 hours. Protein and starch fractions were then separated and analyzed for their (14)C content. At 168 hours, 31% of the total label incorporated in the endosperm was in zein, 15% in glutelin, and 24% in starch. When individual amino acids and sugars in the endosperm powders were analyzed, the (14)C still remaining in the glutamine accounted for only 12 to 14% of the total radioactivity.
已在无菌条件下生长的玉米颖果胚乳组织中研究了作为氮源供应给发育中的玉米胚乳的主要来源谷氨酰胺的命运。在培养系统中,将[U-(14)C]谷氨酰胺包含在培养基中或直接注射到胚乳中。在 168 小时内的不同时间点收获样本。然后分离蛋白质和淀粉部分,并分析其(14)C 含量。在 168 小时时,胚乳中总标记物的 31%存在于醇溶蛋白中,15%存在于谷蛋白中,24%存在于淀粉中。当分析胚乳粉末中的个别氨基酸和糖时,谷氨酰胺中残留的(14)C 仅占总放射性的 12%至 14%。