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灭多威和玉蜀黍平脐蠕孢(小种 T)毒素在解偶联德克萨斯雄性不育细胞质玉米线粒体中的作用。

Mode of Methomyl and Bipolaris maydis (race T) Toxin in Uncoupling Texas Male-Sterile Cytoplasm Corn Mitochondria.

机构信息

Department of Agronomy, University of Illinois, Urbana, Illinois 61801.

出版信息

Plant Physiol. 1985 Apr;77(4):912-6. doi: 10.1104/pp.77.4.912.

Abstract

Bipolaris maydis race T toxin (BmT), and its functional analog, methomyl, uncoupled Texas male-sterile (T) cytoplasm mitochondria by decreasing the resistance of the inner membrane to protons. However, unlike protonophoric or ionophoric agents, BmT toxin and methomyl induced irreversible swelling. Packed volume measurements showed that mitochondrial volume was irreversibly increased by methomyl and BmT toxin indicating that mitochondria no longer functioned as differentially permeable osmometers. The decreased resistance of inner mitochondrial membranes to protons and the loss of osmotic volume regulation suggests that methomyl and BmT toxin induced the formation of hydrophilic pores in T mitochondrial inner membranes.

摘要

玉米弯孢菌 race T 毒素(BmT)及其功能类似物灭多威通过降低内膜对质子的阻力使德州雄性不育(T)细胞质线粒体解偶联。然而,与质子载体或离子载体不同,BmT 毒素和灭多威诱导不可逆肿胀。堆积体积测量表明,灭多威和 BmT 毒素使线粒体体积不可逆增加,表明线粒体不再作为差异渗透性渗透压计发挥作用。内膜对质子的阻力降低和渗透体积调节的丧失表明,灭多威和 BmT 毒素诱导 T 线粒体内膜形成亲水性孔。

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