酸橙盐敏感与离体稳定耐盐细胞系离子积累之间的关系
Relation between Ion Accumulation of Salt-Sensitive and Isolated Stable Salt-Tolerant Cell Lines of Citrus aurantium.
作者信息
Ben-Hayyim G, Spiegel-Roy P, Neumann H
机构信息
Institute of Horticulture, Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel.
出版信息
Plant Physiol. 1985 May;78(1):144-8. doi: 10.1104/pp.78.1.144.
Four selected NaCl-tolerant cell lines of Sour orange (Citrus aurantium) were compared with the nonselected cell line in their growth and internal ion content of Na(+), K(+), and Cl(-) when exposed to increasing NaCl concentrations. No difference was found among the various NaCl-tolerant cell lines in Na(+) and Cl(-) uptake, and all these cell lines took up similar or even larger amounts of Na(+) and Cl(-) than the NaCl-sensitive cell line. Exposure of cells of NaCl-sensitive and NaCl-tolerant lines to equal external concentrations of NaCl, resulted in a greater loss of K(+) from the NaCl-sensitive cell line. This observation leads to the conclusion that growth and ability to retain high levels of internal K(+) are correlated. Exposure of the NaCl-tolerant cell lines to salts other than NaCl resulted in even greater tolerance to Na(2)SO(4), but rather poor tolerance to K(+) introduced as either K(2)SO(4) or KCl; the latter has a stronger inhibitory effect. The NaCl-sensitive cell line proved to be more sensitive to replacement of Na(+) by K(+). Analyses of internal Na(+), K(+), and Cl(-) concentrations failed to identify any particular internal ion concentration which could serve as a reliable marker for salt tolerance.
将四个选定的耐盐酸橙（Citrus aurantium）细胞系与未选定的细胞系在暴露于不断增加的氯化钠浓度时的生长情况以及内部钠离子（Na⁺）、钾离子（K⁺）和氯离子（Cl⁻）含量进行了比较。在不同耐盐细胞系之间，Na⁺和Cl⁻的吸收没有差异，并且所有这些细胞系吸收的Na⁺和Cl⁻量与盐敏感细胞系相似，甚至更多。将盐敏感和耐盐细胞系的细胞暴露于相同外部浓度的氯化钠中，盐敏感细胞系的K⁺损失更大。这一观察结果得出结论，生长与保持高水平内部K⁺的能力相关。将耐盐细胞系暴露于除氯化钠以外的其他盐中，对硫酸钠（Na₂SO₄）的耐受性更强，但对以硫酸钾（K₂SO₄）或氯化钾（KCl）形式引入的K⁺耐受性较差；后者具有更强的抑制作用。盐敏感细胞系被证明对用K⁺替代Na⁺更敏感。对内部Na⁺、K⁺和Cl⁻浓度的分析未能确定任何可作为耐盐可靠标志物的特定内部离子浓度。
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