Federico R, Medda R, Floris G
Dipartimento Biologia Vegetale, Città Universitaria 00185 Roma, Italy.
Plant Physiol. 1985 Jun;78(2):357-8. doi: 10.1104/pp.78.2.357.
Superoxide dismutase has been purified to homogeneity from Lens esculenta cotyledons and shoots. The two forms appeared to be identical. The purified enzyme contained two electrophoretically distinct bands. It contained two ions of Cu and two ions of Zn. Gel filtration experiments indicate a molecular weight of about 33,000. The spectrum of ultraviolet and visible regions and electron paramagnetic resonance were similar to those of Cu-Zn mammalian superoxide dismutase.
超氧化物歧化酶已从蚕豆的子叶和嫩枝中纯化至同质。这两种形式似乎是相同的。纯化后的酶含有两条电泳性质不同的条带。它含有两个铜离子和两个锌离子。凝胶过滤实验表明其分子量约为33,000。紫外和可见光区域的光谱以及电子顺磁共振与铜锌哺乳动物超氧化物歧化酶的相似。
