来自荸荠的超氧化物歧化酶：纯化与性质
Superoxide Dismutase from Lens esculenta: Purification and Properties.
作者信息
Federico R, Medda R, Floris G
机构信息
Dipartimento Biologia Vegetale, Città Universitaria 00185 Roma, Italy.
出版信息
Plant Physiol. 1985 Jun;78(2):357-8. doi: 10.1104/pp.78.2.357.
Abstract
Superoxide dismutase has been purified to homogeneity from Lens esculenta cotyledons and shoots. The two forms appeared to be identical. The purified enzyme contained two electrophoretically distinct bands. It contained two ions of Cu and two ions of Zn. Gel filtration experiments indicate a molecular weight of about 33,000. The spectrum of ultraviolet and visible regions and electron paramagnetic resonance were similar to those of Cu-Zn mammalian superoxide dismutase.
摘要
超氧化物歧化酶已从蚕豆的子叶和嫩枝中纯化至同质。这两种形式似乎是相同的。纯化后的酶含有两条电泳性质不同的条带。它含有两个铜离子和两个锌离子。凝胶过滤实验表明其分子量约为33,000。紫外和可见光区域的光谱以及电子顺磁共振与铜锌哺乳动物超氧化物歧化酶的相似。
相似文献
1
Superoxide Dismutase from Lens esculenta: Purification and Properties.来自荸荠的超氧化物歧化酶：纯化与性质
Plant Physiol. 1985 Jun;78(2):357-8. doi: 10.1104/pp.78.2.357.
2
Purification and properties of Oryza sativa Cu-Zn superoxide dismutase.
Prep Biochem Biotechnol. 1996 May;26(2):135-42. doi: 10.1080/10826069608000059.
3
Purification and Properties of Glyoxysomal Cuprozinc Superoxide Dismutase from Watermelon Cotyledons (Citrullus vulgaris Schrad).瓜类植物绿豆芽乙醛酸循环体铜锌超氧化物歧化酶的纯化和性质（Citrullus vulgaris Schrad）。
Plant Physiol. 1992 Jan;98(1):331-6. doi: 10.1104/pp.98.1.331.
4
Purification and Characterization of Cu, Zn Superoxide Dismutase from Ark Shell Scapharca broughtonii.魁蚶Cu, Zn超氧化物歧化酶的纯化与特性分析
Biosci Biotechnol Biochem. 1998;62(11):2211-6. doi: 10.1271/bbb.62.2211.
5
Purification and properties of cytosolic copper, zinc superoxide dismutase from watermelon (Citrullus vulgaris Schrad.) cotyledons.西瓜（Citrullus vulgaris Schrad.）子叶胞质铜锌超氧化物歧化酶的纯化及性质
Free Radic Res. 1997 Jan;26(1):83-91. doi: 10.3109/10715769709097787.
6
A novel superoxide dismutase from Cicer arietinum L. seedlings: isolation, purification and characterization.来自鹰嘴豆幼苗的一种新型超氧化物歧化酶：分离、纯化及特性分析。
Protein Pept Lett. 2013 Jul 1;20(7):741-8. doi: 10.2174/0929866511320070002.
7
Unique isozymes of superoxide dismutase in Streptomyces griseus.
Arch Biochem Biophys. 1996 Oct 15;334(2):341-8. doi: 10.1006/abbi.1996.0463.
8
The Cu,Zn superoxide dismutases of Aspergillus flavus, Aspergillus niger, Aspergillus nidulans, and Aspergillus terreus: purification and biochemical comparison with the Aspergillus fumigatus Cu,Zn superoxide dismutase.黄曲霉、黑曲霉、构巢曲霉和土曲霉的铜锌超氧化物歧化酶：纯化及其与烟曲霉铜锌超氧化物歧化酶的生化比较
Infect Immun. 1996 Aug;64(8):3326-32. doi: 10.1128/iai.64.8.3326-3332.1996.
9
Purification and properties of superoxide dismutase from Drosophila melanogaster.黑腹果蝇超氧化物歧化酶的纯化及性质
J Biol Chem. 1981 Aug 25;256(16):8506-9.
10
Co(II) derivatives of Cu,Zn-superoxide dismutase with the cobalt bound in the place of copper. A new spectroscopic tool for the study of the active site.铜锌超氧化物歧化酶的钴（II）衍生物，其中钴取代铜的位置结合。一种用于研究活性位点的新光谱工具。
Biochim Biophys Acta. 1984 Mar 29;785(3):111-7. doi: 10.1016/0167-4838(84)90134-1.
引用本文的文献
1
Purification and characterization of thermostable monomeric chloroplastic Cu/Zn superoxide dismutase from Chenopodium murale.从藜科植物中纯化和鉴定热稳定的单体质体 Cu/Zn 超氧化物歧化酶。
Physiol Mol Biol Plants. 2009 Jul;15(3):199-209. doi: 10.1007/s12298-009-0024-8. Epub 2009 Oct 28.
2
Partial purification of superoxide dismutase and peroxidase from ber (Zizyphus mauritiana Lamk.) fruit using anion exchange chromatography.采用阴离子交换层析法从椰枣（蜜枣）果实中部分纯化超氧化物歧化酶和过氧化物酶。
Physiol Mol Biol Plants. 2008 Jul;14(3):167-72. doi: 10.1007/s12298-008-0015-1. Epub 2008 Sep 27.
3
Purification and Properties of Glyoxysomal Cuprozinc Superoxide Dismutase from Watermelon Cotyledons (Citrullus vulgaris Schrad).瓜类植物绿豆芽乙醛酸循环体铜锌超氧化物歧化酶的纯化和性质（Citrullus vulgaris Schrad）。
Plant Physiol. 1992 Jan;98(1):331-6. doi: 10.1104/pp.98.1.331.
本文引用的文献
1
Superoxide dismutases: I. Occurrence in higher plants.超氧化物歧化酶：I. 在高等植物中的存在。
Plant Physiol. 1977 Feb;59(2):309-14. doi: 10.1104/pp.59.2.309.
2
Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein).超氧化物歧化酶。红细胞铜蛋白（血铜蛋白）的酶功能。
J Biol Chem. 1969 Nov 25;244(22):6049-55.
3
An enzyme-based theory of obligate anaerobiosis: the physiological function of superoxide dismutase.基于酶的专性厌氧菌理论：超氧化物歧化酶的生理功能。
Proc Natl Acad Sci U S A. 1971 May;68(5):1024-7. doi: 10.1073/pnas.68.5.1024.
4
Superoxide dismutase: improved assays and an assay applicable to acrylamide gels.超氧化物歧化酶：改进的测定方法及一种适用于丙烯酰胺凝胶的测定方法。
Anal Biochem. 1971 Nov;44(1):276-87. doi: 10.1016/0003-2697(71)90370-8.
5
Superoxide dismutases.超氧化物歧化酶
Adv Enzymol Relat Areas Mol Biol. 1974;41(0):35-97. doi: 10.1002/9780470122860.ch2.
6
Subcellular location of superoxide dismutase in spinach leaves and preparation and properties of crystalline spinach superoxide dismutase.菠菜叶片中超氧化物歧化酶的亚细胞定位以及结晶菠菜超氧化物歧化酶的制备与性质
Eur J Biochem. 1973 Jul 2;36(1):257-66. doi: 10.1111/j.1432-1033.1973.tb02908.x.
7
Preparation and physicochemical properties of green pea superoxide dismutase.绿豆超氧化物歧化酶的制备及其理化性质
Biochim Biophys Acta. 1972 May 12;268(2):305-12. doi: 10.1016/0005-2744(72)90325-7.
Zotero 插件