筛选高光效烟草单倍体。
Selection of Nicotiana tabacum Haploids of High Photosynthetic Efficiency.
机构信息
Instituto Valenciano de Investigaciones Agrarias (I.V.I.A.)-I.N.I.A., Moncada, Valencia, Spain.
出版信息
Plant Physiol. 1985 Oct;79(2):505-8. doi: 10.1104/pp.79.2.505.
Anthers of Nicotiana tabacum cultivar Wisconsin 38 were treated with the mutagen ethyl methane sulfonate and cultured to obtain populations of haploid plants of high genetic variability. The selection of these populations by their photosynthetic efficiency was carried out in a hydroponic culture chamber with a CO(2) atmosphere concentration close to the compensation point. Plants that survived 45 days of treatment were grown in pots in a greenhouse and their performance was compared to a population of unselected haploids. The growth characteristics, net photosynthesis, and chlorophyll content were measured and the haploid character verified. Selected plants were propagated by in vitro culture of buds and then diploidized to obtain seeds. Growth and other characteristics of the plants obtained were compared with those of the parental variety (Wisconsin 38) in a field assay. Growth, dry weight, leaf area, and net photosynthesis of selected plants were higher than in control plants.
将烟草品种威斯康星 38 的花药用诱变剂乙基甲磺酸处理，并进行培养，以获得具有高遗传变异性的单倍体植物群体。通过在接近补偿点的 CO(2)气氛浓度的水培培养室内对这些群体进行光合作用效率的选择。在处理 45 天后存活下来的植物在温室的花盆中生长，并将其性能与未经选择的单倍体群体进行比较。测量了生长特性、净光合作用和叶绿素含量，并验证了单倍体特性。选择的植物通过芽的体外培养进行繁殖，然后二倍体化以获得种子。在田间试验中，将获得的植物的生长和其他特性与亲本品种（威斯康星 38）进行了比较。与对照植物相比，选择植物的生长、干重、叶面积和净光合作用更高。
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