Laboratoire "Echanges cellulaires," UA CNRS 203, Faculté des Sciences, BP 67, 76130 Mont-Saint-Aignan, France.
Plant Physiol. 1985 Oct;79(2):546-51. doi: 10.1104/pp.79.2.546.
The present contribution is devoted to studying the electrical noise of Acer pseudoplatanus cells in culture suspensions. Spontaneous voltage noise of the cells was recorded by means of a microelectrode inserted in the vacuole. The small signal impedance of the cell was measured so that it was possible to study the intensity spectra of the noise. We recorded intensity spectra with cells incubated in 10(-3) molar gramicidin A. Difference spectra showed characteristics of a channel noise. By using the calculated conductance of gramicidin A in an artificial membrane, and by simplifying assumptions for the ionic transports through plasmalemma and tonoplast, we were able to estimate the electrochemical potential difference for K(+) ions across the plasmalemma (3.2 +/- 1 millivolt).
本研究致力于研究 Acer pseudoplatanus 细胞在培养悬浮液中的电噪声。通过插入液泡中的微电极记录细胞的自发电压噪声。测量细胞的小信号阻抗，以便研究噪声的强度谱。我们记录了在 10（-3）摩尔短杆菌肽 A 孵育的细胞的强度谱。差谱显示了通道噪声的特征。通过使用人工膜中短杆菌肽 A 的计算电导，并简化穿过质膜和液泡膜的离子运输的假设，我们能够估计穿过质膜的 K（+）离子的电化学势差（3.2 +/- 1 毫伏）。
