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类黄酮的形成作为豆类(菜豆)叶片紫外线胁迫的指标:在评估因太阳紫外线-B 辐射增加而导致的潜在损伤时,光修复的意义。

Isoflavonoid Formation as an Indicator of UV Stress in Bean (Phaseolus vulgaris L.) Leaves : The Significance of Photorepair in Assessing Potential Damage by Increased Solar UV-B Radiation.

机构信息

Biologisches Institut II, Universität Freiburg, Schänzlestrasse 1, D-7800 Freiburg, West Germany.

出版信息

Plant Physiol. 1985 Nov;79(3):630-4. doi: 10.1104/pp.79.3.630.

Abstract

Induction of the isoflavonoid pigment, coumestrol (3,9-dihydroxy-6H-benzofuro-[3,2-c][1] benzopyran-6-one), in primary leaves of beans (Phaseolus vulgaris L. var Saxa) by ultraviolet (UV) radiation was used as a quantifiable marker for UV damage to a plant system. Coumestrol was induced only by wavelengths below 300 nanometers and its formation could be reversed by treatment with white, but not red light after the UV irradiation period. Formation of coumestrol by UV could also be prevented over a period of 14 hours by simultaneous irradiation with blue light provided that the blue fluence rate was high enough. The results suggest that coumestrol formation is mediated via UV-induced pyrimidine dimer formation in the plant DNA and the photorepair properties of blue light are discussed with respect to possible increases in solar UV due to stratospheric ozone depletion.

摘要

紫外(UV)辐射诱导菜豆(Phaseolus vulgaris L. var Saxa)初生叶片中异黄酮色素香豆雌酚(3,9-二羟基-6H-苯并呋喃-[3,2-c][1]苯并吡喃-6-酮)的产生,被用作植物系统中 UV 损伤的可量化标记。香豆雌酚仅由波长低于 300 纳米的光诱导产生,并且在 UV 照射期后,用白色光(而非红光)处理可以逆转其形成。在 14 小时的时间内,通过同时用蓝光辐照也可以防止 UV 诱导的香豆雌酚形成,只要蓝光的通量率足够高。结果表明,香豆雌酚的形成是通过植物 DNA 中 UV 诱导的嘧啶二聚体形成介导的,并且讨论了蓝光的光修复特性,因为平流层臭氧消耗可能会增加太阳 UV。

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