Mg-原卟啉 IX 单甲酯（氧化）环化酶体系的性质。
Properties of the Mg-Protoporphyrin IX Monomethyl Ester (Oxidative) Cyclase System.
机构信息
Department of Botany, University of California, Davis, California 95616.
出版信息
Plant Physiol. 1985 Nov;79(3):730-3. doi: 10.1104/pp.79.3.730.
Abstract
Mg-protoporphyrin IX monomethyl ester (oxidative) cyclase, the enzyme system responsible for the formation of the chlorophyll isocyclic ring, exhibits requirements for both essential sulfhydryls and essential disulfides. It is inhibited by N-ethylmaleimide, dithiothreitol, and beta-mercaptoethanol, but not by sodium arsenite. This enzyme system shows some substrate specificity: (a) the 6-side-chain of the macrocycle can either be a methyl propionate ester, or its beta-hydroxy or beta-keto derivatives; (b) the 7-side-chain can either be a propionic acid or a methyl propionate ester; (c) both the 4-vinyl and the 4-ethyl series can serve as substrates, at least at the beta-keto ester level; (d) the activity appears to be lost if the side-chain in the 2-position is reduced from a vinyl to an ethyl.
摘要
镁原卟啉 IX 单甲酯（氧化）环化酶，负责形成叶绿素的环状结构的酶系统，表现出对必需巯基和必需二硫键的需求。它被 N-乙基马来酰亚胺、二硫苏糖醇和β-巯基乙醇抑制，但不受亚砷酸钠抑制。该酶系统表现出一些底物特异性：（a）大环的 6-侧链可以是甲酯，或者是其β-羟基或β-酮衍生物；（b）7-侧链可以是丙酸或甲酯；（c）4-乙烯基和 4-乙基系列都可以作为底物，至少在β-酮酯水平上可以；（d）如果 2-位的侧链从乙烯基还原为乙基，则活性似乎会丧失。
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本文引用的文献
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2
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3
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