United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland 20705.
Plant Physiol. 1986 Jan;80(1):14-9. doi: 10.1104/pp.80.1.14.
Indole-3-acetic acid (IAA) labeled with (13)C in the six carbons of the benzene ring is described for use as an internal standard for quantitative mass spectral analysis of IAA by gas chromatography/selected ion monitoring. [(13)C(6)]IAA was compared to the available deuterium labeled compounds and shown to offer the advantages of nonexchangeability of the isotope label, high isotopic enrichment, and chromatographic properties identical to that of the unlabeled compound. The utility of [(13)C(6)]IAA for measurement of endogenous IAA levels was demonstrated by analysis of IAA in Lemna gibba G-3.
吲哚-3-乙酸(IAA)在苯环的 6 个碳原子上标记(13)C,用于作为气相色谱/选择离子监测定量质谱分析 IAA 的内标。[(13)C(6)]IAA 与现有的氘标记化合物进行了比较,结果表明其具有同位素标记不可交换性、高同位素丰度和与未标记化合物相同的色谱性质等优点。通过分析浮萍 G-3 中的 IAA,证明了 [(13)C(6)]IAA 用于测量内源性 IAA 水平的实用性。