采用氘代类似物和选择离子监测法测定花旗松中的吲哚-3-乙酸：幼苗和成年树中微量的比较。
Determination of Indole-3-acetic Acid in Douglas Fir Using a Deuterated Analog and Selected Ion Monitoring: Comparison of Microquantities in Seedling and Adult Tree.
机构信息
Department of Chemistry and Biochemical Sciences, Oregon Graduate Center, Beaverton, Oregon 97005.
出版信息
Plant Physiol. 1978 Dec;62(6):841-5. doi: 10.1104/pp.62.6.841.
Abstract
Indole-3-acetic acid (IAA) content in shoot tips of Douglas fir (Pseudotsuga menziesii [Mirb.] Franco) trees and seedlings was determined by combined gas chromatography-mass spectrometry using a deuterated analog (d(2)-IAA) as an internal standard and the technique of selected ion monitoring. Ratios of the peak heights of the deuterated analog internal standard to endogenous IAA revealed a slightly higher content of IAA in seedlings compared with the shoot tips collected in June. The relatively high level of IAA (2.9 micrograms per gram fresh weight) in seedlings is discussed in relation to in vitro propagation of this species.
摘要
采用氘代类似物（d(2)-IAA）作为内标，通过选择离子监测技术，结合气相色谱-质谱法测定了花旗松（Pseudotsuga menziesii [Mirb.] Franco）树木和幼苗茎尖中的吲哚-3-乙酸（IAA）含量。氘代内标峰高与内源性 IAA 峰高的比值表明，与 6 月采集的茎尖相比，幼苗中的 IAA 含量略高。幼苗中相对较高水平的 IAA（每克鲜重 2.9 微克）与该物种的体外繁殖有关。
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本文引用的文献
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Regeneration of douglas fir plantlets through tissue culture.通过组织培养再生道格拉斯冷杉的植株。
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Reversible inactivation of aged solutions of indolyl-3-acetic Acid.吲哚乙酸溶液的衰老作用可逆失活。
Science. 1966 Dec 9;154(3754):1327-9. doi: 10.1126/science.154.3754.1327.
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