United States Department of Agriculture, Agricultural Research Service, State University Station, Fargo, North Dakota 58105.
Plant Physiol. 1986 Mar;80(3):612-7. doi: 10.1104/pp.80.3.612.
The antagonistic interaction between the grass herbicide, diclofopmethyl (methyl 2-[4(2',4'-dichlorophenoxy)phenoxy]propanoate) (DM), and 2,4-dichlorophenoxyacetic acid (2,4-D), was demonstrated in DM-resistant soybean (Glycine max [L.] Merr.) and DM-susceptible corn (Zea mays L.). 2,4-D caused root shortening and thickening, and induced callus growth in soybean and corn root tissue cultures at 1 and 10 micromolar. Normal soybean root growth was unaffected by 10 micromolar DM whereas corn root growth was inhibited completely by 1 to 10 micromolar DM. DM at 10 micromolar reversed completely the induction of callus growth by 1 micromolar 2,4-D in soybean roots. In corn, 10 micromolar 2,4-D reversed the growth inhibiting activity of 1 micromolar DM and induced callus growth. The antagonistic interaction between DM and 2,4-D was reciprocal and the activity of either compound depended upon the relative concentration of the other. 2,4-D did not antagonize or decrease the activity of DM by decreasing its uptake by root tissues or increasing the rate of its detoxication. The antagonistic interaction between DM and 2,4-D probably involves involves cellular activity associated with actively growing and proliferating cells and requires the presence of both compounds at the sensitive site.
草甘膦（甲基 2-[4(2',4'-二氯苯氧基)苯氧基]丙酸）（DM）与 2,4-二氯苯氧乙酸（2,4-D）之间的拮抗相互作用在抗草甘膦大豆（Glycine max [L.] Merr.）和草甘膦敏感玉米（Zea mays L.）中得到了证实。2,4-D 在 1 和 10 微摩尔时会导致大豆和玉米根组织培养物的根缩短和变粗，并诱导愈伤组织生长。10 微摩尔的 DM 不会影响正常大豆根的生长，而 1 至 10 微摩尔的 DM 则完全抑制玉米根的生长。10 微摩尔的 DM 完全逆转了 1 微摩尔 2,4-D 对大豆根愈伤组织生长的诱导作用。在玉米中，10 微摩尔的 2,4-D 逆转了 1 微摩尔 DM 的生长抑制活性，并诱导愈伤组织生长。DM 和 2,4-D 之间的拮抗相互作用是相互的，并且两种化合物的活性取决于另一种化合物的相对浓度。2,4-D 并没有通过降低根组织对 DM 的吸收或增加其解毒速度来拮抗或降低 DM 的活性。DM 和 2,4-D 之间的拮抗相互作用可能涉及与活跃生长和增殖细胞相关的细胞活动，并且需要两种化合物都存在于敏感部位。
