Darr S C, Arntzen C J
MSU/DOE Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824.
Plant Physiol. 1986 Apr;80(4):931-7. doi: 10.1104/pp.80.4.931.
Conditions were developed to isolate the light-harvesting chlorophyll-protein complex serving photosystem II (LHC-II) using a dialyzable detergent, octylpolyoxyethylene. This LHC-II was successfully reconstituted into partially developed chloroplast thylakoids of Hordeum vulgare var Morex (barley) seedlings which were deficient in LHC-II. Functional association of LHC-II with the photosystem II (PSII) core complex was measured by two independent functional assays of PSII sensitization by LHC-II. A 3-fold excess of reconstituted LHC-II was required to equal the activity of LHC developing in vivo. We suggest that a linker component may be absent in the partially developed membranes which is required for specific association of the PSII core complex and LHC-II.
利用可透析去污剂辛基聚氧乙烯，开发了分离用于光系统II的捕光叶绿素蛋白复合物（LHC-II）的条件。该LHC-II成功地重组到大麦品种Morex（大麦）幼苗部分发育的叶绿体类囊体中，这些幼苗缺乏LHC-II。通过LHC-II对光系统II（PSII）致敏的两种独立功能测定法，测量了LHC-II与光系统II（PSII）核心复合物的功能关联。需要3倍过量的重组LHC-II才能与体内发育的LHC活性相当。我们认为，部分发育的膜中可能缺少PSII核心复合物与LHC-II特异性结合所需的连接成分。
