Frey S M, Urban J E
Division of Biology, Ackert Hall, Kansas State University, Manhattan, Kansas 66506.
Plant Physiol. 1986 May;81(1):326-8. doi: 10.1104/pp.81.1.326.
The number of nodules produced per clover seedling inoculated with Rhizobium trifolii 0403 can be increased almost 2-fold by the addition of penicillin or mecillinam. Two-day-old dutch white clover seedlings grown in 250 milliliter boston round jars containing agar-solidified plant growth medium were inoculated with exponentially growing Rhizobium trifolii 0403 cells. Penicillin or mecillinam (100 micrograms per milliliter) were added immediately or after 24 hours. Following 42 days growth, 10 replicate sets of 5 plants for each treatment were assayed for nodule number, plant dry weight, and Kjeldahl nitrogen. Both antibiotics increased nodule number, plant dry weight, and Kjeldahl nitrogen. Increases in nodule number and dry weight were statistically significant. The range of values in Kjeldahl nitrogen was so extensive as to make the data insignificant at the P < 0.05 level, however nodule number, plant dry weight, and Kjeldahl nitrogen displayed a significant correlation with each other. There were no significant differences in treatment with either antibiotic or with time of treatment. Nodule number increased by about 85%, and plant dry weight and nitrogen increased by about 30%.
用三叶草根瘤菌0403接种的每株三叶草幼苗产生的根瘤数量，通过添加青霉素或美西林可增加近2倍。在含有琼脂固化植物生长培养基的250毫升波士顿圆瓶中生长的2日龄荷兰白三叶草幼苗，接种指数生长的三叶草根瘤菌0403细胞。立即或在24小时后添加青霉素或美西林（每毫升100微克）。生长42天后，对每种处理的10组、每组5株植物进行根瘤数、植物干重和凯氏定氮分析。两种抗生素均增加了根瘤数、植物干重和凯氏定氮量。根瘤数和干重的增加具有统计学意义。凯氏定氮值的范围非常广泛，以至于在P < 0.05水平时数据无统计学意义，然而根瘤数、植物干重和凯氏定氮量之间显示出显著的相关性。两种抗生素处理或处理时间之间均无显著差异。根瘤数增加约85%，植物干重和氮含量增加约30%。
