Newton R J, Bhaskaran S, Puryear J D, Smith R H
Department of Forest Science, Texas Agricultural Experiment Station, Texas A&M University, College Station, Texas 77843.
Plant Physiol. 1986 Jun;81(2):626-9. doi: 10.1104/pp.81.2.626.
Eight cultivars Sorghum bicolor (L.) Moench were grown as callus cultures under induced, prolonged water stress (8 weeks), with polyethylene glycol in the medium. Concentrations of soluble carbohydrates and organic acids in callus were measured at the end of the growth period to determine differences in response to prolonged water stress. Sucrose, glucose, fructose, and malate were the predominant solutes detected in all callus at all water potentials. All cultivars had high levels of solutes in the absence of water stress and low levels in the presence of prolonged water stress. However, at low water potentials, low levels of solutes were observed in drought-tolerant cultivar callus and high solute levels were observed in drought-susceptible cultivar callus. Estimated sucrose concentrations were significantly higher in water-stressed, susceptible cultivar callus. Large solute concentrations in susceptible cultivar callus were attributed to osmotic adjustment and/or reduced growth during water stress.
八个高粱品种[高粱（Sorghum bicolor (L.) Moench）]在含有聚乙二醇的培养基中，于诱导的长期水分胁迫（8周）下进行愈伤组织培养。在生长周期结束时测量愈伤组织中可溶性碳水化合物和有机酸的浓度，以确定对长期水分胁迫的响应差异。蔗糖、葡萄糖、果糖和苹果酸是在所有水势下所有愈伤组织中检测到的主要溶质。所有品种在无水分胁迫时溶质水平较高，在长期水分胁迫时溶质水平较低。然而，在低水势下，耐旱品种愈伤组织中溶质水平较低，而干旱敏感品种愈伤组织中溶质水平较高。水分胁迫下敏感品种愈伤组织中的估计蔗糖浓度显著更高。敏感品种愈伤组织中溶质浓度较高归因于水分胁迫期间的渗透调节和/或生长减缓。
