Institute of Biological Sciences, University of Tsukuba, Sakura-mura Ibaraki, 305 Japan.
Plant Physiol. 1986 Jul;81(3):931-3. doi: 10.1104/pp.81.3.931.
Glycoproteins released from carrot cells into culture media were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and visualized by staining with Coomassie brilliant blue or with the periodic-acid Schiff procedure. The appearance or disappearance of two glycoproteins of M(r) 65,000 (GP65) and M(r) 57,000 (GP57) was closely related to the formation of somatic embroys. GP65 was released specifically from embryogenic cells cultured in a medium without 2,4-dichlorophenoxyacetic acid, in which they can form somatic embryos. GP57 was released from the same embryogenic cells, if they were cultured in a medium with 2,4-dichlorophenoxyacetic acid, in which they cannot form somatic embryos. Nonembryogenic cells which cannot form somatic embryos, released only GP57.
从胡萝卜细胞释放到培养基中的糖蛋白通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳进行分析，并通过考马斯亮蓝染色或过碘酸希夫（periodic-acid Schiff）程序进行可视化。两种糖蛋白 M(r)65,000（GP65）和 M(r)57,000（GP57）的出现或消失与体细胞胚胎的形成密切相关。GP65 仅从在不含 2,4-二氯苯氧乙酸的培养基中培养的胚胎发生细胞中释放，在该培养基中它们可以形成体细胞胚胎。GP57 从相同的胚胎发生细胞中释放，如果它们在含有 2,4-二氯苯氧乙酸的培养基中培养，在该培养基中它们不能形成体细胞胚胎。不能形成体细胞胚胎的非胚胎发生细胞仅释放 GP57。
