Identification of endogenous gibberellins from sorghum.
机构信息
Department of Biological Sciences, University of Lethbridge, Lethbridge, Alberta T1K 3M4, Canada.
出版信息
Plant Physiol. 1986 Sep;82(1):330-2. doi: 10.1104/pp.82.1.330.
Abstract
Gibberellins (GA) A(1), A(19), and A(20) were identified in shoot cylinders containing the apical meristems from sorghum (Sorghum bicolor L.). Extracts were purified by sequential SiO(2) partition chromatography and reversed-phase C(18) high performance liquid chromatography and biologically active (dwarf rice cv Tan-ginbozu microdrop assay) fractions were subjected to gas chromatography-selected ion monitoring. Based on the use of [(3)H]GA and (2)HGA internal standards, amounts of GA(1), GA(19), and GA(20) were estimated to be 0.7, 8.8, and 1.5 namograms per gram dry weight of tissue, respectively.
摘要
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