Department of Vegetable Crops, University of California, Davis, California 95616.
Plant Physiol. 1986 Dec;82(4):925-9. doi: 10.1104/pp.82.4.925.
Since CO(2) is known to stimulate ethylene production by promoting the conversion of 1-aminocyclopropane-1-carboxylic acid (ACC) to ethylene, the effect of CO(2) on the activity and the development of the ethylene forming enzyme (EFE) was studied in tobacco (Nicotiana tabacum L. cv Havana 425 and Xanthi) leaf discs. In addition to previous observations that EFE activity is dependent on CO(2) concentration and is saturable with 2% CO(2), present data show two saturation curves at 2% and 10% CO(2). Promotion of EFE development was dependent also on CO(2) concentration (saturated at 2% CO(2)) and duration (maximum at 24 in the dark), and was abolished by 20 micromolar cycloheximide. Application of exogenous ethylene (20 microliters per liter) or light treatment further increased the CO(2)-enhanced development of EFE, implying that these two factors can also affect EFE development via interaction with CO(2). The results suggest that CO(2) exerts its stimulatory effect on the conversion of ACC to ethylene by enhancing not only the activity but also the synthesis of EFE in leaf discs.
由于二氧化碳已知通过促进 1-氨基环丙烷-1-羧酸（ACC）向乙烯的转化来刺激乙烯的产生，因此研究了二氧化碳对烟草（Nicotiana tabacum L. cv Havana 425 和 Xanthi）叶圆片中的乙烯形成酶（EFE）的活性和发育的影响。除了先前观察到的 EFE 活性依赖于二氧化碳浓度并且可以被 2%的二氧化碳饱和之外，目前的数据还显示在 2%和 10%的二氧化碳下有两条饱和曲线。EFE 发育的促进也依赖于二氧化碳浓度（在 2%的二氧化碳下饱和）和持续时间（在黑暗中 24 小时达到最大值），并且被 20 微摩尔环己酰亚胺所消除。外源乙烯（每升 20 微升）的应用或光处理进一步增加了二氧化碳增强的 EFE 发育，这意味着这两个因素也可以通过与二氧化碳的相互作用来影响 EFE 的发育。结果表明，二氧化碳通过增强叶片圆片中 EFE 的活性和合成，不仅对 ACC 向乙烯的转化产生了刺激作用。
