Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824.
Plant Physiol. 1987 Jan;83(1):1-3. doi: 10.1104/pp.83.1.1.
A competitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for the rapid quantitation of the glycosylated and deglycosylated forms of the monomeric soluble extensin precursor subunits P1 and P2. A log-linear response range for each kind of precursor in the competition curve was between 0.01 and 100 nanograms per milliliter.
建立了一种竞争性间接酶联免疫吸附测定法(ELISA),用于快速定量单体可溶性伸展素前体亚基 P1 和 P2 的糖基化和去糖基化形式。在竞争曲线中,每种前体的对数线性响应范围在 0.01 至 100 纳克/毫升之间。